The following are the basic troubleshooting steps which should positively resolve this kind of issue.

• Use the USB cable that came with your remote as some USB cables may not work correctly.
• Check that the USB cable is securely connected directly to your computer.
• Do not use USB hubs.
• Try using a different USB port on your computer.
• Confirm that the USB cable is connected to your remote properly.
• Try rebooting your computer.
• Try rebooting the Harmony remote by holding the power button for 15 seconds.
• Try setting up your remote using a different computer.

If the issue persists, we’d request you to follow the below steps in reinstalling the Harmony drivers which should fix this issue:

• Open the “Run” dialog box by pressing and holding the Windows key, then press the R key (“Run”).
• Type devmgmt.msc and click OK.
• Click on the box to the left of "Human Interface Devices" to expand the list. You'll be able to view the "USB Input Device".
• Double click on that and you will be able to find a driver with “Location: Location 0 (Port_#0004.Hub_#0003)” which is Harmony driver.
• Click on Uninstall to uninstall the driver.
• Once uninstalled, unplug the remote from USB and plug it again. The USB drivers will be installed automatically and Windows will be able to detect your remote.